Time-lapse confocal imaging of calcium dynamics in starfish embryos

Article Abstract:

Calcium dynamics in starfish embryos during prefertilization maturation, fertilization and cleavage was marked by absence of noticeable transients, a single nonoscillating wave and repetitive oscillations, respectively, suggesting that starfish early development can include changes in the spatiotemporal patterns of calcium spiking. For analysis using time-lapse confocal microscopy, oocytes were injected with dextran-conjugated forms of the calcium-sensitive fluorochrome calcium green and the calcium-insensitive dye rhodamine. Injections of colchicine and heparin were also used to evaluate the role played by calcium fluxes.

Calcium in the body, Calcium (Nutrient), Starfishes

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Cadherin-11 expressed in association with mesenchymal morphogenesis in the head, somite, and limb bud or early mouse embryos

Article Abstract:

A study was conducted to examine the expression and function of cahedrin, a molecule which plays an important role in cell adhesion, by cloning mouse cad-11 cDNA. In situ hybridization of early mouse embryos revealed cad-11 in the mesodermal layers in the head and tail regions and the mid-to-late primitive streak stages. However, somitization restricted the expression of cad-11 to sclerotome cells and limited its appearance at the peripheral mesenchyme. These results suggest that cad-11 is involved in selective association of mesenchymal cells.

Cell adhesion molecules, Cell adhesion

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Development of chemosensitivity in serotonin-deficient spinal cords of rat embryos

Article Abstract:

Researchers blocked the synthesis of 5-hydroxytryptamine (5-HT) and examined the motoneuron reactions to exogenous 5-T one or two weeks later to ascertain whether serotonin (5-hydroxytryptamine, 5-HT) in the developing projections causes the modulation of alterations in neuronal reactions to 5-HT. 5-HT was not essential for receptor expression in growing spinal neurons, but it could be the cause for receptor down-regulation.

Abnormalities, Influence, Rats, Animal anatomy, Serotonin, Nerves, Spinal, Spinal nerves

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