Two-dimensional polyacrylamide gel electropherosis analysis of the acid tolerance response in Listeria monocytogenes LO28

Article Abstract:

The two-dimensional polycrylamide gel electrophoresis analysis of the acid tolerance response in Listeria monocytogenes LO20 was examined to describe alterations in protein levels on L. monocytogenes during acid habituation. The study used a two-dimensional SDS-polyacrylamide gel electrophoresis to examine protein alterations during acid adaptation. Results reveal alterations in protein synthesis during acid adaptation in L. monocytogenes that can lead to increased chances of survival under stressful conditions.

Research, Listeria monocytogenes, Acid deposition

---

Rapid real-time PCR assay for detection and quantitation of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk

Article Abstract:

A real-time PCR assay with primers and probes designed by using IS900, which allowed rapid detection of Mycobacterium avium subsp. paratuberculosis DNA in artificially contaminated milk was developed using fluorescence energy transfer technology and Lightcycler analysis. The entire procedure was performed in less than 3 hours and was successfully adapted to quantify M. avium subsp. paratuberculosis in spiked milk from heavily and mildly contaminated samples.

Diagnosis, Causes of, Technology application, Energy transfer, Milk contamination

---

Peptide aMptD-mediated capture PCR for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples

Article Abstract:

A peptide-mediated capture PCR was developed and characterized for detection of Mycobacterium avium subsp. paratuberculosis in bulk milk samples using peptide aMptD. This method depends solely on synthetic reagents, does well under routine laboratory conditions and is adaptable to standard laboratory automation.

Physiological aspects, Johne's disease

Similar abstracts:

• Abstracts: Effect of food processing-related stresses on acid tolerance of Listeria monocytogenes
• Abstracts: Two-component transcriptional regulation of N-acyl-homoserine lactone production in Pseudomonas aureofaciens. Fate of the biological control agent Pseudomonas aureofaciens TX-1 after application to turfgrass
• Abstracts: Molecular and genetic analysis of the toxic effect of RAP1 overexpression in yeast. The clr1 locus regulates the expression of the cryptic mating-type loci of fission yeast
• Abstracts: DNA extraction and PCR methods for the detection of Listeria monocytogenes in cold-smoked salmon. Development of a PCR-enzyme immunoassay oligoprobe deection method for Toxoplasma gondii oocysts, incorporating PCR controls
• Abstracts: The robustness of the transcriptional response to alterations in morphogenetic gradients

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.