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Use of PMAI as a housekeeping biomarker for assessment of toxic-induced stress in Saccharomyces cerevisiae

Article Abstract:

Toxicant induced stress of pure compounds in Saccharomyces cerevisiae utilizing a destabilized version of green fluorescent protein optimized for expression in yeast (yEGFP3) under control of the promoter of the housekeeping membrane ATPease gene PMAI were studied. It demonstrated the versatility of the novel biomarker to complement existing test batteries to assess contaminant exposure and effects.

Author: Schmitt, Marcel, Schwanewilm, Petra, Ludwig, Jost, Lichtenberg-Frate, Hella
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Ecological stress

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Effects of ADH2 overexpression in Saccharomyces bayanus during alcoholic fermentation

Article Abstract:

The effect of overexpression of the gene ADH2 on metabolic and biological activity in Saccharomyces bayanus V5 during alcoholic fermentation is examined. The increased contents in acetoin, acetic acid, and L-proline present in the fermentation medium is attributed to detoxification by removal of excess acetaldehyde and the need to restore and maintain the intracellular redox potential balance.

Author: Maestre, Oscar, Garcia-Martinez, Teresa, Peinado, Rafael A., Mauricio, Juan C.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
Evaluation, Fermentation, Gene expression, Redox potential

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Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations

Article Abstract:

The transferability of this respiratory phenotype to the V5hxt1-7delta strain a derivative of a strain used in enology was demonstrated. Even the more relevant for the potential use of such a strain for the production of heterologous protein and also of low-alcohol beverages is the observation that the biomass yield increased 50% with the mutant compared to its parental strain.

Author: Henricsson, C., Bill, R.M., Ferreira, Jesus M.C. de, Norbeck, J., Haefalk, K., Hohmann, S., Elbing, K., Gustafsson, L., Larsson, C.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Phenotype, Phenotypes, Biomass

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Subjects list: Environmental aspects, Genetic aspects, Brewer's yeast, Saccharomyces cerevisiae, Research
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