Use of a glycerol-limited, long-term chemostat for isolation of Escherichia coli mutants with improved physiological properties

Article Abstract:

The evolution of an Escherichia coli MG1655 population from a single clone was analyzed in a glycerol-limited thermostat to characterize their growth physiology and stress resistance. Analysis of the Escherichia coli microbial culture exhibited two different colony morphologies that evolved from the original wild type MG1655 strain under glucose-limited continuous cultivation. The clones from one of the transformed population also have higher growth rates, biomass yields and better stress resistance compared to the wild-type strain.

Escherichia coli, Microbial growth

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Powerful methods to establish chromosomal markers in Lactococcus lactis: an analysis of pyrimidine salvage pathway mutants obtained by positive selections

Article Abstract:

Studies of mutants of Lactococcus lactis lacking pyrimidine and purine genes reveal that the one pyrimidine nucleoside phosphorylase is responsible for the cleavage of uridine, deoxyuridine and thymidine. A purine nucleoside phosphorylase cleaves ribonucleoside and deoxyribonucleoside derivatives of adenine, guanine and hypoxanthine. Lactococcus lactis has pathways for the synthesis of nucleotides and can grow in a medium without nucleosides and nucleotides.

Observations, Phosphorylase

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Mutations in the glycerol kinase gene restore the ability of ptsGHI mutant of Bacillus subtilis to grow on glycerol

Article Abstract:

The ability of Bacillus subtilis to consume and metabolize glycerol was found to be affected by mutations in the sequence coding for its phosphotransferase system. This genes for this system is associated with the sequence for the synthesis of glycerol kinase. Mutant strains of Bacillus subtilis that were unable to grow in glycerol medium were found to contain nonsense codons within the glycerol kinase sequence.

Glycerol kinase

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