Use of fluorescence resonance energy transfer for rapid detection of enteroviral infection in vivo

Article Abstract:

A novel fluorescent-based detection method for identifying enteroviral infections is reported by using fluorescence resonance energy transfer (FRET) between a donor (enhanced cyan fluorescent protein [ECFP]) and an acceptor (enhanced yellow fluorescent protein [EYFP]) fluorophore joined by a linker peptide containing the proteolytic site of 2[A.sup.pro]. This method is applicable for other viruses portraying similar characteristics.

Diagnosis, Enterovirus diseases, Enterovirus infections, Microbiological techniques (Diagnosis)

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Freezing induces biased results in the molecular detection of Flavobacterium columnare

Article Abstract:

Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Since freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR and the detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish.

Commercial Fishing, Fishing, Usage, Fishes, Electron microscopy

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PCR-based detection of angiostrongylus cantonensis in tissue and mucus secretions from molluscan hosts

Article Abstract:

A PCR-based method was developed to detect Angiostrongylus cantonensis directly from molluscan tissue to control the outbreak of human eosinophilic meningitis.

Genetic aspects, Mollusks, Eosinophils, Molluscs

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