Visualization and detection of infectious coxsackievirus replication using a combined cell culture-molecular beacon assay

Article Abstract:

A generalized method is developed for the rapid and sensitive detection of infectious viruses from cell culture by directly visualizing newly synthesized viral RNA with molecular beacons (MBs). This method, when combined with nuclease-resistant MBs, is useful not only for real-time detection of infectious viruses but also to assess the life cycle of viral processing in vitro.

United States, Genetic aspects, Virus diseases, Cell culture, Coxsackieviruses

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Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos

Article Abstract:

The directed evolution of organophosphorus hydrolase (OPH) to improve the hydrolysis of a poorly hydrolyzable substrate, chlopyrifos is described. The results indicate that the directed evolution of OPH and related enzymes may lead to potential biological solutions for efficient decontamination and detection of neurotoxic nerve agents.

All Other Basic Organic Chemical Manufacturing, Industrial Organic Chemicals, Organophosphorous Compounds, Diagnosis, Neurotoxic agents, Hydrolysis, Organophosphorus compounds, Organic phosphorus compounds

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Real-time nuclei acid sequence-based amplification assay for detection of hepatitis A virus

Article Abstract:

The development of an molecular beacons (MB)-based real-time nuclei acid sequence-based amplification (NASBA) assay for the detection of hepatitis A virus is investigated. The NASBA assay successfully detected as few as 10 PFU from seeded lake water samples when combined with immunomagnetic separation.

Other Lighting Equipment Manufacturing, Lighting equipment, not elsewhere classified, Marine Beacons, Beacons & Marker Lighting, Usage, Hepatitis A virus, Hepatovirus, Beacons, Nucleobases

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