Xenopus CDC7 function is dependent on licensing but not on XORC, XCdc6, or CDK activity and is required for XCdc45 loading

Article Abstract:

Xenopus CDC7 function has been found to be dependent on licensing, but not on XCdc6, XORC, or CDK activity. It is required for XCdc45 loading. The role of XCdc7 in DNA replication is described. Sequential binding of the origin recognition complex (ORC), Cdc6, and the minichromosome maintenance (MCM/P1) proteins gives a licensed replication origin. Cell-free extracts of Xenopus eggs were used for the study. SCdc7 was seen to bind to chromatin in G1 and S phases. CDK activity is not required for association of XCdc7 with chromatin. A simple model has been found for the assembly of functional initiation complexes in the Xenopus system.

DNA, Carrier proteins, Transport proteins, Protein kinases, Chromosomes

---

Xenopus Sprouty2 inhibits FGF-mediated gastrulation movements but does not affect mesoderm induction and patterning

Article Abstract:

Xenopus Sprouty2 has been found to inhibit fibroblast growth factor (FGF)-mediated gastrulation movements but not to affect mesoderm induction/patterning. FGF receptor signal transduction is necessary for specifying the body plan of a vertebrate. The Drosophila gene sprouty encodes an antagonist of FGF signaling. Xenopus sprouty2 has been cloned. It is expressed in a pattern like that of known FGFs. Results of study give evidence for at least two FGF-dependent signal transduction pathways.

Vertebrates, Cellular signal transduction, Mesoderm, Gastrulation, Fibroblast growth factors

---

p27(super.Xic1), a Cdk inhibitor, promotes the determination of glial cells in Xenopus retina

Article Abstract:

A Cdk inhibitor which promotes determination of glial cells in Xenopus retina is discussed. It is p27(super.Xic1) and a member of the Cip/Kip family of inhibitors. It inhibits cell division and induces Muller glia from retinoblasts. Expression of the inhibitor increases gradually in retinal development. It is first obvious in gastrula stages as diffuse staining all through the epidermis.

Developmental biology, Retina, Cell differentiation, Neuroglia, Glia

Similar abstracts:

• Abstracts: Ras pathway specificity is determined by the integration of multiple signal-activated and tissue-restricted transcription factors
• Abstracts: Functionally distinct, sequence-specific replicator and origin elements are required for Drosophila chorion gene amplification
• Abstracts: Synergistic regulation of vertebrate muscle development by Dach2, Eya2, and Six1, homologs of genes required for Drosophila eye formation
• Abstracts: Mismatch repair-dependent processing of methylation damage gives rise to persistent single-stranded gaps in newly replicated DNA
• Abstracts: Chk1 is an essential kinase that is regulated by Atr and required for the G(sub.2)/M DNA damage checkpoint

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.