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Amino acid specificity of glycation and protein-AGE crosslilnking reactivities determined with a dipeptide SPOT library

Article Abstract:

Changes in protein conformation are caused by advanced glycation end products (AGEs), as well as loss of function and irreversible crosslinking. Using a library of dipeptides on cellulose membranes (SPOT library) an approach to systematically assay the relative reactivities of amino acid side chains and the N-terminal amino group to sugars and protein-AGEs were developed.

Author: Munch, Gerald et al
Publisher: Nature Publishing Co.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 1999
Germany

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A fluorescent indicator for visualizing aAMP-induced phosphorylation in vivo

Article Abstract:

Researchers have developed a method for visualizing phosphorylation of proteins in living cells utilizing an innovative fluorescent indicator made up of two green fluorescent protein (GFP) variants connected by the kinase-inducible domain (KID) of the transcription factor cyclic adenosine monophosphate (cAMP)-responsive element binding protein (CREB).

Author: Nagai, Yasuo et al
Publisher: Nature Publishing Co.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2000
Japan, All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Fluorescent Chemicals ex Dyes, Fluorescent chemicals

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Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells

Article Abstract:

Researchers from the Karolinska Institutet, Stockholm, Sweden developed a green flourescent protein that allows the measurement of proteolysis in living cells.

Author: Dantuma, Nico P., Lindsten, Kristina, Glas, Rickard, Jellne, Marianne, Masucci, Maria G.
Publisher: Nature Publishing Co.
Publication Name: Nature Biotechnology
Subject: Business
ISSN: 1087-0156
Year: 2000
Sweden

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Subjects list: Cellular proteins
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