Directed evolution of adeno-associated virus yields enhanced gene delivery vectors

Article Abstract:

A study in which a directed evolution approach involving the generation of large mutant capsid libraries and selection of adeno-associated virus (AAV) 2 variants with enhanced properties was applied. The high-throughput selection processes were designed to isolate mutants within the library with altered affinities for heparin or the ability to evade antibody neutralization and deliver genes more efficiently and wild-type capsid in the presence of anti-AAV serum.

California, Heparin Preparations, Physiological aspects, Gene expression, Adenoviruses, Heparin, Properties

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RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy

Article Abstract:

The silencing of codeinone reductase (COR) in opium poppy, Papaver somniferum, which was obtained by using a hairpin RNA construct designed to silence the members of the multigene COR family through ribonucleic acid interference (RNAi), is presented. The results indicate that the RNAi could be responsible for the replacement of morphine with the nonnarcotic alkaloid reticuline and result in the high-yield accumulation of reticuline.

Morphine (Unit Dose), Morphine, Opium poppy, RNA interference

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Antidote-mediated control of an anticoagulant aptamer in vivo

Article Abstract:

It is demonstrated that the matched antidotes are used to control the activity and side-effects of aptamers. The antidote rapidly reverse anticoagulation engendered by the drug and prevent drug-induced bleeding in surgically challenged animals, which demonstrates that rationally designed drug-antidote pairs are generated to provide control over drug activities in animals.

United States, Anticoagulant Preparations, Care and treatment, Coronary heart disease, Anticoagulants (Medicine), Anticoagulants, Antidotes

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