F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses

Article Abstract:

Laser-assisted injection of embryonic stem (ES) cells into eight cell-stage embryos has generated viable and healthy F0 generation mice that contain no more than 0.1% host contamination. This method uses both inbred and hybrid ES cells with either inbred or outbred host embryos and also works not only with standard male XY ES cells, but also with their XO cogenic clones, thus yielding male and female F0 founder mice.

United States, Gene mutations, Gene mutation, Genetic aspects, Technology application, Embryonic stem cells

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Baculovirus expression system for heterologous multiprotein complexes

Article Abstract:

A simple and versatile method for producing recombinant baculovirus deoxyribonucleic acid (DNA), which is used for expressing protein complexes that contain many subunits, is described. Studies show that this system can also be used for recombinant multiprotein production and multigene transfer applications.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Recombinant proteins

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Baculovirus as versatile vectors for protein expression in insect and mammalian cells

Article Abstract:

Baculovirus infected insect cells and mammalian cells are found to be capable of producing thousands of recombinant proteins, ranging from cytosolic enzymes to membrane-bound proteins. Details of the isolation and quantification of recombinant baculovirus and its use in genetic engineering are discussed.

Nucleic acids, Nucleic acid isolation

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