Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery

Article Abstract:

The infectivity of integrase-defective lentiviral vectors (IDLV) is used for expressing zinc-finger nucleases (ZFNs) and providing the template DNA for gene correction in different cell types. IDLV delivery and ZFNs targeting distinct loci are used for observing high levels of gene addition in a panel of human cell lines, as well as human embryonic stem cells, allowing rapid, selection-free isolation of clonogenic cells with the desired genetic modification.

Gene expression, Stem cell research

---

Specificity by design

Article Abstract:

The specificity of a homing endonuclease is altered, using computational modeling of the protein-DNA interface. In the past few years there was great progress made in the design and selection of variant C2H2 zinc-finger proteins that can be targeted to be almost any desired site in the DNA.

Analysis, Genetic aspects, Cloning, Protein research, DNA-ligand interactions, DNA binding

---

An improved zinc-finger nuclease architecture for highly specific genome editing

Article Abstract:

Two advanced zinc-finger nucleases using structure-based design which give high gene-modification efficiencies only when paired as a heterodimer with reduced levels of genome-wide cleavage are presented.

DNA repair

Similar abstracts:

• Abstracts: Efficient gene silencing with high selectivity using mission shRNA lentiviral vectors. RISC assessment
• Abstracts: Banking market structure, financial dependence and growth: international evidence from industry data. Banks' advantage in hedging liquidity risk: theory and evidence from the commercial paper market
• Abstracts: A process model of strategic business exit: implications for an evolutionary perspective on strategy. Let chaos reign, then rein in chaos - repeatedly: managing strategic dymanics for corporate longevity
• Abstracts: Decoupling risk taking from income stream uncertainty: a holistic model of risk. CEO incentives, innovation, and performance in technology-intensive firms: a reconciliation of outcome and behavior-based incentive schemes
• Abstracts: A two-for-one in tomato nutritional enhancement. Loosening lignin's grip on biofuel production. Lignin modification improves fermentable sugar yields for biofuel production

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.