Time-resolved fluorescence of flavin adenine dinucleotide in wild-type and mutant NADH peroxidase. Elucidation of quenching sites and discovery of a new fluorescence depolarization mechanism

Article Abstract:

A study was conducted to analyze the flavin adenine dinucleotide of tetrameric NADH peroxidase from Enterococcus faecalis and three mutant enzymes using time-resolved polarized fluorescence. The energy transfer between the flavins in various subunits within dimeric flavoproteins were determined by time-resolved fluorescence anisotropy analysis ofthe bound flavin cofactor. Results indicated that fluorescence lifetime heterogeneity was not limited to glutathione reductase.

Enzymes, Peroxidase, Glutathione

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Anchoring effects of self-assembled monolayers for polymer-dispersed liquid crystals films

Article Abstract:

Fabrication of the PDLC films of 5CB using the SAM-modified substrates to explore effects of the surface modifications for the PDLC films is presented. The molecular aggregation manner of 5CB in the interface layer is highly dependent on the chemical nature as well as the polarity of the SAMs deposited onto the substrate surfaces.

Science & research, Observations, Surface chemistry, Substrates

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Steady-state and time-resolved fluorescence analysis for a cyanobiphenyl mesogen in polymer-dispersed liquid crystal films

Article Abstract:

Polymer-dispersed liquid crystal (PDLC) films were examined for fluorescence behavior and compared with 4-cyano-4'-pentylbiphenyls (5CB) having no polymer component.

Polymer liquid crystals, Liquid crystalline polymers

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