Two-dimensional attenuated total reflection/infrared correlation spectroscopy of adsorption-induced and concentration-dependent spectral variations of beta-lactoglobulin in aqueous solutions

Article Abstract:

The potential of generalized two-dimensional (2D) attenuated total reflection/infrared (ATR/IR) spectroscopy in studies of spectral variations in the amide I region of aqueous solutions of protein are demonstrated. The 2D map reveals that the intensity changes observed for the series of aqueous solutions of beta-lactoglobulin with different concentrations are predominantly composed of concentration-induced secondary structure changes.

Amides

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Two-dimensional/attenuated total reflection infrared correlation spectroscopy studies on secondary structural changes in human serum albumin in aqueous solutions: pH-dependent structural changes in the secondary structures and in the hydrogen bondings of side chains

Article Abstract:

The acid unfolding process of human serum albumin (HSA) by use of a generalized two-dimensional attenuated total reflection infrared correlation spectroscopy. The Two-dimensional correlation spectra have yielded information of secondary structural changes and changes in the COO(super -) and COOH groups and other side chains.

Analysis, Usage

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Two-dimensional near-infrared spectroscopy study of human serum albumin in aqueous solutions: using overtones and combination modes to monitor temperature-dependent changes in the secondary structure

Article Abstract:

FT-NIR spectra were measured for human serum albumim (HSA) in aqueous solutions with concentrations of 1.0, 2.0, 3.0, 4.0 and 5.0 wt % over a temperature range of 45-80 degree celsius. Results reveal that the overtone and combination modes are very useful in monitoring subtle changes in protein dynamics.

Chemical reactions, Spectra (Spectroscopy)

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