An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

Article Abstract:

The post-transcriptional element of double-stranded RNA-dependent gene silencing seems to be carried out by a sequence-specific nuclease incorporating these small RNAs as guides that target specific messages based on sequence recognition. S2 cells were transfected with a double-stranded RNA corresponding to the first 540 nucleotides of Drosophila cyclin E, a gene that is vital for progression into S phase of the cell cycle, in order to establish whether RNA interference could be used to target endogenous genes. It is now vital to establish whether conservation of gene-silencing mechanisms exists at the transcriptional level.

Suppression, Genetic, Suppressor mutation (Molecular genetics), Genetic transcription, Transcription (Genetics)

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Role for a bidentate ribonuclease in the initiation step of RNA interference

Article Abstract:

The 'dicer' enzyme has been identified that can produce putative guide RNAs.

Enzymes

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A microRNA polycistron as a potential human oncogene

Article Abstract:

B-cell lymphoma samples and cell lines to normal tissues are compared and reveal that the levels of the primary or mature microRNAs derived from the mir 17-92 locus are often substantially increased in cancer. Enforced expression of the mir-17-92 cluster acted with c-myc expression to accelerate tumor development in a mouse B-cell lymphoma model.

United States, Science & research, Development and progression, Gene expression, Lymphomas, Oncogenes

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