Analysis of a RanGTP-regulated gradient in mitotic somatic cells

Article Abstract:

The Ran-importin-(beta) system in cells was examined by conventional and fluorescence lifetime microscopy using a biosensor, termed Rango, that increases its fluorescence resonance energy transfer signal when released from importin-(beta) by RanGTP. The results indicate that conserved Ran-regulated pathways are involved in multiple, parallel processes required for spindle, function, but their relative contribution differs in chromatin versus centrosome/kinetochore-driven spindle assembly systems.

Analysis, Fluorescence microscopy, Somatic cells, Energy transformation

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Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts

Article Abstract:

Bipolar mitotic spindles assemble around DNA-coated beads incubated in Xenopus egg extracts in the absence of centrosomes and kinetochores. A cytoplasmic dynein-dependent translocation of microtubules across one another allows the spindle formation. The bipolar microtubules assembling around chromatin in a mitotic cytoplasm rearrange into arrays of uniform polarity. The rearrangement follows the nucleation of microtubules at dispersed sites with random polarity.

Observations, Xenopus, Microtubules, Spindle (Cell division), Spindle (Cytoplasm)

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Cells on chips

Article Abstract:

The application of microtechnology to cell biology with emphasis on microsystems aimed at gaining biological insight, as well as on efforts to realize increasing cell handling integration and biochemical analysis levels on chips is discussed. Methods for cell colure, regulation of extracellular cues, cell fractionations and biochemical analysis on a micrometre scale are outlined.

Massachusetts, Usage, Technology application, Cell research, Cytological research, Tissue culture, Chip sets (Computers), Chipsets, Chipset

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