DNA shuffling of a family of genes from diverse species accelerates directed evolution

Article Abstract:

DNA shuffling is significant in directed evolution, generating diversity through recombination. Previous studies have concentrated on single genes and random point mutations, and an alternative source of diversity is naturally occurring homologous genes, providing functional diversity. The efficiency of obtaining moxalactamase activity from cephalosporinase genes is compared to that obtained from a mixed pool of genes. Single cycle shuffling yielded eightfold improvements from the separate genes, compared to a 270-540-fold improvement from the mixed genes.

Physiological aspects, DNA, Observations, Genetic recombination

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Waves, particles and fullerenes

Article Abstract:

It has been established that molecules of the fullerene C60 have wave as well as particle properties, just as suggested by quantum theory. It was possible to prove the interference of the de Broglie waves connected with molecules of the fullerence C60. This research extends the applicability of wave-particle duality by around one order of magnitude in the macroscopic direction.

Research, Carbon allotropes

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