Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1
Article Abstract:
GTP-hydrolysing proteins, called Rho GTPases, regulate the actin cytoskeleton and act as molecular switches in various signal-transduction processes. Rho GTPases can be inactivated by different bacterial toxins by glucosylation or ADP-ribosylation. Previous research shows that Rho proteins are assumed targets for Escherichia coli cytotoxic necrotizing factors 1 and 2, which trigger multinucleation and actin assembly in culture cells. A study showing that a glutamine residue at position 63 (Gln 63) of Rho is deamidated by E. coli cytotoxic necrotizing factor 1 is presented.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells
Article Abstract:
Researchers have succeeded in showing that DNA ligase IV co-immunoprecipitates with XRCC4. These two proteins interact with each other in a yeast two-hybrid system. It is clear that XRCC4 plays a role in DNA metabolism, being required to stimulate DNA ligase activity, mainly through direct structure interaction with DNA ligase IV. It is likely that DNA ligase IV is the joining activity in V(D)J recombination.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
Article Abstract:
A new technique for sequencing proteins isolated by polyacrylamide gel electrophoresis is proposed. The method uses nano-electrospray tandem mass spectrometry. The technique is around 10-100 times more sensitive, faster and requires less purification and manipulation steps than other existing techniques. It is expected to become the method of choice for protein microcharacterization.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Engineering Escherichia coli to see light. Nutrients in remote mode. Nutrients in synergy
- Abstracts: Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex. Crystal structure of the p27(sup Kip1) cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex
- Abstracts: Shocking states of matter. Solid oxygen takes shape. Turning off the water
- Abstracts: Soldier production under threat. Increased soldier production in ant colonies exposed to intraspecific competition
- Abstracts: Regulation of glutamate release by presynaptic kainate receptors in the hippocampus. Induction of LTP in the hippocampus needs synaptic activation of glutamate metabotropic receptors