Mapping of the porcine Colipase gene to chromosome 7 using linkage analysis

Article Abstract:

The Colipase (CLPS) gene found in pigs was mapped to chromosome 7 using linkage analysis. The restriction endonuclease DdeI was used to detect using polymerase chain reaction a restriction fragment length polymorphism of CLPS. It is an essential co-factor in lipase and is a vital regulator of metabolism in dietary lipids in pigs. CLPS together with enterostatin regulates swine body weight by controlling appetite and fat digestion.

Methods

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Rapid communication: the porcine inter-alpha trypsin inhibitor-heavy chain 4 (ITIH4) gene maps to chromosome 13

Article Abstract:

The restriction endonuclease AciI was used to detect a restriction fragment length polymorphism within PCR amplification products of the porcine inter-alpha-trypsin inhibitor-heavy chain 4 (ITIH4) gene. A significant linkage from the ITIH4 gene to several microsatellite markers on porcine chromosome 13 was found. Further, the ITIH4 gene was linked to several other genes including the transferin and cholecystokinin loci.

Genetic polymorphisms

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Rapid communication: restriction fragment length polymorphism in amplification products of the porcine growth hormone-releasing hormone gene

Article Abstract:

Gene sequencing analysis of the porcine growth hormone-releasing hormone gene revealed a significant similarity with portions of exon 3 of the same gene in humans and rats. This was revealed through PCR amplification of the gene using the restriction enzyme AluI. A poplymorphism with two alleles was detected in the agarose gel solution. Linkage relationships between the gene and previously mapped loci were revealed.

Analysis, Polymerase chain reaction

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