Cystic fibrosis in the mouse by targeted insertional mutagenesis

Article Abstract:

A new mouse model for cystic fibrosis (CF) has been devised that should be useful in trials of potential treatments for this deadly autosomal recessive disorder. The new model was produced through mutagenesis or the genetic disruption of the CF transmembrane conductance regulator gene in murine embryonic stem cells. The detection of the characteristic CF pathophysiology in the mice such as abnormal chloride ion transport verified the similarity of the model to human CF.

Models, Usage, Innovations, Cystic fibrosis, Mice as laboratory animals, House mouse, Mutagenesis

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Magnetic bead capture of expressed sequences encoded within large genomic segments

Article Abstract:

Biotin-streptavidin magnetic bead technology can be used effectively to isolate complementary DNA (cDNAs) encoded in large genomic segments. Conventional methods are inefficient when the genomic interval is greater than several hundred thousand base pairs. Magnetic bead technology may be used to enrich cDNA encoded over very large segments such as the candidate gene for Huntington's disease.

Research, Evaluation, Equipment and supplies, DNA probes, Genomes, Genetic research

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