Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein

Article Abstract:

A model system is used in which the bacterial fitness has correlated with the enzymatic activity of TEM-1 [beta]-lactamase, an enzyme that degrades the antibiotic ampicillin and thereby confers ampicillin resistance on Gram-negative bacteria such as Escherichia coli. The threshold robustness is found to be inherently epistatic and once the stability threshold is exhausted, the deleterious effects of mutations become fully pronounced, thereby making proteins far less robust than generally assumed.

Israel, Polymerase chain reaction, Beta lactamases, Genetic epistasis, Epistasis

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Synthesis and properties of crosslinked recombinant pro-resilin

Article Abstract:

The cloning and expression of the first exon of the Drosophila CG15920 gene as a soluble protein in Escherichia coli is reported. The resilience of crosslinked recombinant resilin is found to exceed that of unfilled synthetic polybutadiene, a high resilience rubber.

Plastics Material and Resin Manufacturing, Plastics materials and resins, Polybutadiene Resins, Radiation, Radiation (Physics), Mechanical properties, Polybutadienes

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Crystal structures of a multidrug transporter reveal a functionally rotating mechanism

Article Abstract:

AcrB is a principal multidrug efflux transporter in Escherichia coli that cooperates with an outer-membrane channel, ToIC, and a membrane-fusion protein, AcrA. A short description on crystal structures of AcrB with and without substrates is presented.

Membrane proteins, Crystals, Crystal structure, Substrates

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