Stoichiometry and turnover in single, functioning membrane protein complexes

Article Abstract:

The protein stoichiometry, dynamics and turnover of the MotB, a component of the stator that couples ion flow to torque generation and anchors the stator to the cell wall, with single molecular precision in functioning bacterial flagellar motors in Escherichia coli is investigated. The study represents the first instance of direct measurement of the number and rapid turnover of protein subunits within a functioning molecular machine.

Membrane proteins, Stoichiometry, Torque

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Structure of the E. coli protein-conducting channel bound to a translating ribosome

Article Abstract:

A cryo-electron microscopy reconstruction of the Escherichia coli protein-conducting channel (PCC), SecYEG, complexed with the ribosome and a nascent chain containing a signal anchor is presented. A model for co-translational protein translocation is proposed on the basis of the observation in the translocating PCC of two segregated pores with different degrees of access to bulk lipid.

Analysis, Electron microscopy, Ribosomal proteins, Properties, Structure

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Direct observation of steps in rotation of the bacterial flagellar motor

Article Abstract:

A stepping motion of a Na(super +)-driven chimaeric flagellar motor in Escherichia coli at low sodium-motive force and with controlled expression of a small number of torque-generating units is demonstrated. Twenty-six steps per revolution are observed, which is consistent with the periodicity of the ring of FliG protein, the proposed site of torque generation on the rotor.

Flagella (Microbiology), Flagella, Animal locomotion

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