A simple, efficient method for the separation of humic substances and DNA from environmental samples

Article Abstract:

A research was conducted to study the use of different gels for the removal of humic contaminants from DNA extracts of environmental samples. The environmental samples used were woodland soil, hydrocarbon-contaminated soil, aquatic sediment and aquatic biofilm. Aliquots of each sample were loaded onto column types while effluents were collected after centrifugation. Results showed sepharose 4B spin columns to be capable of purifying DNAs from a variety of aquatic and terrestrial habitats.

DNA, Colloids, Microbial contamination

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Arsenite-oxidizing Hydrogenobaculum strain isolated from an acid-sulfate-chloride geothermal spring in Yellowstone National Park

Article Abstract:

An arsenite-oxidizing Hydrogenobaculum strain is isolated from a geothermal spring that was previously shown to contain microbial populations engaged in arsenite oxidation. The isolate is sensitive to both arsenite and arsenate and behaves as an obligate chemolithoautograph that uses H2 as its sole energy source and has an optimum temperature of 55 to 60 degrees and an optimum pH of 3.0.

Genetic aspects, Microbial populations, Genetic research

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New insights into microbial oxidation of antimony and arsenic

Article Abstract:

The microbial oxidation of antimony (Sb) and arsenic (As) are studied. It is shown that Sb(III) oxidation is catalyzed by a pathway that is different from that used for As(III) and they both have the ability to oxidize metalloids.

Primary nonferrous metals, not elsewhere classified, Primary Smelting and Refining of Nonferrous Metal (except Copper and Aluminum), Antimony, Analysis, Physiological aspects, Polymerase chain reaction, Oxidation-reduction reaction, Oxidation-reduction reactions, Agrobacterium tumefaciens, Chemical properties

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