The nitric oxide regulated nor promoter of Paracoccus denitrificans

Article Abstract:

The promoter of the Paracoccus denitrificans nitric oxide (NO) reductase operon (norCBQDEF) has been studied using primer extension and deletion analysis. Two transcription-start sites were seen, the major one active only in cultures grown anaerobically, with nitrate, and is 45.5 bp downstream of the putative NNR-binding site, so this is a class II factor-dependent promoter. NNR is the sole regulatory protein with a significant role in regulating expression of the NO reductase. The NNR-binding site has been confirmed.

United Kingdom, Bacteria, Genetic regulation, Microbial enzymes, Genetic transcription, Transcription (Genetics), Nitric oxide, Binding sites (Biochemistry), Active sites (Biochemistry), Denitrification

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Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration

Article Abstract:

Green fluorescent protein (GFP) fluorescence in Streptococcus gordonii was assessed under low-oxygen concentration and low-pH conditions. GFP, it was found, can be used in studies of oxygen-tolerant anaerobic bacteria. Densely packed, flow cell-grown biofilms of S. gordonii do not develop oxygen gradients that inhibit GFP fluorescence development. The transient nature of GFP fluorescence in acid-producing bacteria can often be overcome in flowcells.

United States, Denmark, Oxygen, Streptococcus, Bacterial growth, Microbial mats, Fluorescence, Anaerobic bacteria, Gram-positive bacteria, Hydrogen-ion concentration

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