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Abstracts » Biological sciences

Broad-host-range shuttle vectors for screening of regulated promoter activity in viridans group streptococci: isolation of a pH-regulated promoter

Article Abstract:

Results demonstrate that the pH-regulated promoter of Streptococcus gordonii enables the bacterium to sense changes in the environmental pH and turn on the gene activation mechanism. Data further reveal that such cue-regulated vectors are useful in research on adapttive gene expression in viridans group of streptococci.

Author: Vriesema, Aldwin J.M., Brinkman, Rene, Kok, Jan, Dankert, Jacob, Zaat, Sebastian A.J.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
Statistical Data Included, Streptococcus, Bacteria, Pathogenic, Pathogenic bacteria, Gene expression, Promoters (Genetics)

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Identification and characterization of two novel clostridial bacteriocins, Circularin A and Closticin 574

Article Abstract:

Results describe characterization of the new antibacterial peptides isolated from clostridial species in terms of their purification, structural genes encoding the peptides, and N-terminal sequence of the peptides. Data indicate that closticin 574 is an 82-amino-acid bacteriocin, while circularin A is produced as a propeptide of 72 amino acids.

Author: Kok, Jan, Kemperman, Rober, Kuipers, Anneke, Karsens, Harma, Nauta, Arjen, Kuipers, Oscar
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Bacteriostats, Antibacterial agents, Clostridium

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Functional analysis of the gene cluster involved in production of the bacteriocin circularin A by Clostridium beijerinckii ATCC 25752

Article Abstract:

Research demonstrates that a 12 kilobase region of the antibacterial peptide circularin A structural gene encodes the proteins involved in the production and secretion of the peptide in Clostridium beijerinckii ATCC 25752. Data indicate that a minimum of five genes are required for the expression of circularin A and that an ATP-binding CirD protein together with CirB and CirC produce the bacteriocin.

Author: Kuipers, Oscar P., Kok, Jan, Kemperman, Rober, Nauta, Arjen, Jonker, Marnix
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Antiinfective Preparations, Anti-infective agents, Genetic aspects, Microbiological synthesis, Bacteriocins

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Subjects list: Research, Analysis, Netherlands, Physiological aspects, Bacterial proteins
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