Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in the yeast

Article Abstract:

A galactose-inducible expression library is constructed by cloning yeast cDNA molecules under GAL1 regulation. From this base, eleven independent librarie were created with individual average size of 1x10(super 6) clones. The libraries measure 50 times as much as the mRNA population in a yeast cell. The library was tested for identifying genes whose overexpression causes lethality in yeast. Clones that indicated lethality included cDNAs encoding structural proteins, genes in signal transduction pathways and cDNAs overexpressing nonhistone protein B and nuclear sequence recognition protein.

Usage, Cloning, Galactose

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Intermolecular transposition of IS10 causes coupled homologous recombination at the transposition site

Article Abstract:

Two plasmids of inactive IS10 sequences are coupled during transposition to cointegrate indicating that transposition results in duplication of the IS10. The Tn10 ends of bacterial transposon are inverted repeats of the insertion sequence IS10. Tn10 and IS10 can transpose by a nonreplicative mechanism involving "cut and paste". IS10-right is a functional insertion sequence that codes the protein transposase which can cause Tn10 and IS10 transposition and other rearrangement.

Analysis, Observations, Plasmids, Translocation (Genetics), Transposons

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Positive and negative regulatory elements control expression of the yeast retrotransposon Ty3

Article Abstract:

Researchers studied the Ty3 transcription and identification of Ty3 areas that mediate pheromone and mating-type control to direct its expression with the yeast life cycle. A group of strains was developed which was isogenic save for the number of Ty3 elements, which differed from zero to three. Ty3 expression studies revealed that each of the three elements was transcribed and controlled.

Research, Retroviruses

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