Contribution of methanotrophic and nitrifying bacteria to CH4 and NH4+ oxidation in the rhizosphere of rice plants as determined by new methods of discrimination

Article Abstract:

Two approaches were developed for estimating the relative contributions of methanotrophs to NH4+ oxidation and of nitrifiers to CH4 oxidation in soil planted with rice plants. These new options for discrimination between the activities of methanotrophs and nitrifiers, which are based on the competitive inhibitor CH3F and on recovery after inhibition with C2H2, will also assess the role of methanotrophs and nitrifiers in nitrogen and carbon cycling in soils and sediments. The competitive exclusion assay and the differential recovery assay were found to be applicable to soil slurries, but similar approaches may prove useful for assessing the contributions of methanotrophs and nitrifiers to CH4 and NH4+ turnover in situ.

Physiological aspects, Rice, Oxidation-reduction reaction, Oxidation-reduction reactions, Rhizosphere

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Microelectrode measurements of the activity distribution in nitrifying bacterial aggregates

Article Abstract:

The application of the fluidized-bed process technique in the treatment of wastewater is discussed. Nitrifying aggregates were grown in a fluidized-bed tractor and were studied using ammonium, oxygen and nitrate microelectrodes andacidity profiles. Oxygen penetration depth determined activity distribution. The necessity of accompanying oxygen microelectrodes with ammonium or nitrate microelectrodes was demonstrated.

Measurement, Sewage microbiology, Microelectrodes

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Effects of aeration cycles on nitrifying bacterial populations and nitrogen removal in intermittently aerated reactors

Article Abstract:

The effects of the lengths of aeration and nonaeration periods on nitrogen removal and the nitrifying bacterial community structure are investigated in intermittently aerated (IA) reactors treating digested swine wastewater. The results have shown that superior ammonia removal efficacies are not associated with levels of specific ammonia-oxidizing bacteria (AOB) species or with higher AOB species diversity.

United States, Science & research, Methods, Sewage treatment

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