Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR

Article Abstract:

A new two-step filtration protocol followed by real-time PCR assay is built up to directly quantitate salmonellae in two types of biological samples that is chicken rinse and spent irrigation water. Four prefiltration filters, one type of final filter, and six protocols for recovery of salmonellae from the final filter are evaluated to identify an effective filtration protocol and this study has contributed to the development of a simple, rapid and reliable method for qauntitation of salmonellae.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Polymerase chain reaction

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Nontyphoidal Salmonellae in United Kingdom badgers: prevalence and spatial distribution

Article Abstract:

Results reveal that 18 of the 25 badger social groups excrete different nontyphoidal Salmonella species, which exhibit susceptibility to antimicrobials. Data indicate that the S. enterica serovar Agama and S. enterica serovar Binza isolates are similar, whereas S. enterica serovar Lomita and S. enterica serovar Ried are different. Furthermore, S. enterica serovar Agama are spatially clustered.

United Kingdom, Speciation, Dachshund, Dachshunds

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Sensitivity of planktonic and biofilm-associated Salmonella spp. to ionizing radiation

Article Abstract:

Salmonella on a variety of foods and contact surfaces is inactivated using ionizing radiation. The radiation sensitivity of planktonic or biofilm-associated cells is determined for three food-borne-illness-associated isolates of Salmonella.

United States, Observations, Microbial mats, Planktonic bacteria

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