Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion

Article Abstract:

The deacetoxycephalosporin C synthase from Streptomyces clavuligerus is directly modified for enhancement of penicillin G expansion into phenylacetyl-7-aminodeacetoxycephalosporanic acid. The analysis has provided promising enzymes for cephalosporin biosynthesis along with a large number of mutants, which has provided new insights into the structure-function relationship of the protein that should lead to further rational engineering.

United States, Streptomyces, Microbiological chemistry, Chemical properties

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Family shuffling of expandase genes to enhance substrate specificity for penicillin G

Article Abstract:

Screening of several new expandase genes from actinomycetes and enhancing the substrate specificity for penicillin G using DNA family shuffling is reported. The evolution of penicillin G-specific expandase was successfully directed and the results indicate the remarkable plasticity of the expandase, suggesting that more-active chimeras might be achievable with further rounds.

Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Microbiological research

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Engineering streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G

Article Abstract:

Three mutants each by random- and site-directed mutagenesis of the Streptomyces clavuligerus show two- to sixfold and 6- to 14-fold increase in the catalysis rate of deacetoxycephalosporin C enzyme activity than that of wild-type, respectively. Furthermore, double and triple mutants show even higher enhancement of enzyme activity and reactivity toward pencillin G.

Taiwan, Product information, Pharmaceutical Preparation Manufacturing, Pharmaceutical preparations, Cephalosporin Preparations, Analysis, Testing, Physiological aspects, Catalysis, Mutagenesis, Cephalosporins, Ring formation (Chemistry), Cyclization (Chemistry), Moxalactam, Cephaloridine

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