Gene cloning, nucleotide sequencing, and purification and characterization of the low-specificity L-threonine aldolase from Pseudomonas sp. strain NCIMB 10558

Article Abstract:

Site-directed mutagenesis was used to investigate the cloning, sequencing and overexpression in Escherichia coli cells of the low-specificity L-threonine aldolase (L-TA) gene from the Pseudomonas strain NCIMB 10558, purification and characterization of the recombinant enzyme and the identification of the active-site lysine residue of the enzyme. The amino acid sequence displayed less than 20% identity with those of low-specificity L-TA from Saccharomyces cerevisiae, L-allo-threonine aldolase from Aeromonas jandaei and other hypothetical proteins from other microorganisms.

Usage, Genetic aspects, Escherichia coli, Enzymes, Pseudomonas, Mutagenesis, Amino acid sequence, Amino acid sequencing, Threonine

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Production of dihomo-gamma-linolenic acid by a delta5-desaturase-defective mutant of Mortierella alpina 1S-4

Article Abstract:

A delta 5-desaturase-defective mutant, termed Mut44, of the fungus Mortierella alpina 1S-4 was found to exhibit high mycelial dihomo-gamma-linolenic acid (DHGA) activity. The mutant grew in a simple medium with significant DHGA productivity. Various factors affecting the DHGA production were the glucose and yeast extract concentrations and growth temperature. There were no problems, however, with the inhibitors present in oils. Most of the DHGA was present in the triglyceride fraction among lipid classes.

Linolenic acids, Linolenic acid, Fatty acid desaturases

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Production of 5,8,11-eicosatrienoic acid (mead acid) by a delta6 desaturation activity-enhanced mutant derived from a delta12 desaturase-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4

Article Abstract:

A novel Mortierella alpina mutant (M209-7) derived from the delta12 desaturase-defective Mut48 strain produced greater amounts of 5,811-eicosatrienoic acid (mead acid) compared to its parent strain. The M209-7 strain was similar to Mut48 in morphology, glucose consumption, total fatty acid composition and growth. Furthermore, optimization of cultivation temperature and glucose feeding enhanced the synthesis of mead acid by the mutant Mortierella alpina M209-7 strain.

Microbial metabolism, Microbiological synthesis

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