In vivo Himar1-based transposon mutagenesis of Francisella tularensis

Article Abstract:

The utilization of a conditionally replicating derivative for delivery of a modified Himar1 (HimarFT) into Francisella tularensis live vaccine strain (LVS) in vivo is reported. The results suggest that a comprehensive library of mutants could be generated in Francisella tularensis LVS, providing an additional genetic tool to identify virulence determinants required for survival within the host.

Methods, Genetic aspects, Transposons, Pathogenic microorganisms, Mutagenesis

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Discrimination between Francisella tularensis and Francisella-like endosymbionts when screening ticks by PCR

Article Abstract:

A study is conducted to show that tick pools initially positive for Francisella tularensis based on a 16S rRNA gene PCR assay are determined to be positive only for Francisella-like endosymbionts. The need for careful evaluation of PCR-based diagnostic in laboratories that screen ticks for Francisella tularensis is reported.

Analysis, Polymerase chain reaction, Endosymbiosis

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Methods of enhanced culture recovery of francisella tularensis

Article Abstract:

Improved recovery times and a sensitivity of 90 percent of francisella tularensis is demonstrated when cultures are inoculated using fresh tissues. Here, comparisons are also performed using samples from a tularemia outbreak in prairie dogs.

Tissues, Tissues (Anatomy)

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