Increasing the carbon flux toward synthesis of short-chain-length-medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle

Article Abstract:

The short-chain-length-medium-chain-length polyhydroxyalkanoates are synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. Using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenasse activity towards R-3-hydroxybutyryl coenzyme A increased the quantity of polymer produced.

Genetic aspects, Gene expression

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Modification of the monomer composition of polyhydroxyalkanoate synthesized in Saccharomyces cerevisiae expressing variants of the beta-oxidation-associated multifunctional enzyme

Article Abstract:

The monomer composition of medium-chain-length polyhydroxyalkanoate (MCL-PHA) produced in Saccharomyces cerevisiae peroxisomes through the manipulation of the enzymes of the core beta-oxidation cycle is modified. Results indicate that it was possible to modify the PHA monomer composition through an increase in the proportion of the short-chain monomers of five and six carbons.

Nutritional aspects, Monomers

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Synthesis of polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae by using intermediates of fatty acid beta-oxidation

Article Abstract:

Saccharomyces cerevisiae can be genetically modified to produce medium-chain polyhydroxyalkanoate (PHA). PHA is of interest because it is a biodegradable polyester. The genetic modification includes the transformation of S. cerevisiae with the PHAC1 synthase from Pseudomonas aeruginosa targeted to the peroxisome.

Microbial genetic engineering, Polyesters, Industrial microbiology

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