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Involvement of two specific causes of cell mortality in freeze-thaw cycles with freezing to -196 degrees Celsius

Article Abstract:

A study is conducted to examine cell viability after freezing. Two distinct ranges of temperature were identified as corresponding to stages at which yeast cell mortality occurred during freezing to minus196 degrees Celsius, the upper range related to the temperature of crystallization of the medium and the lower temperature range related to recrystallization that occurred during thawing.

Author: Dumont, Frederic, Marechal, Pierre-Andre, Gervais, Patrick
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Genetic aspects, Yeast fungi, Yeasts (Fungi), Freezing points, Freezing

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Cell size and water permeability as determining factors for cell viability after freezing at different cooling rates

Article Abstract:

Research examines the viabilities of different cell types, such as yeast cells, bacterial cells, and human cells, with respect to their cooling rate from 5 and 30,000 C per minute. Results show a U-pattern for the cell viability versus cooling rate. All cell types show an higher viability at low and very high cooling rates.

Author: Dumont, Frederic, Marechal, Pierre-Andre, Gervais, Patrick
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
France, Influence, Cells (Biology), Cells, Thermal properties, Cryobiology, Cooling

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Synergistic and antagonistic effects of combined subzero temperature and high pressure on inactivation of Escherichia coli

Article Abstract:

A study was conducted on the combined effects of subzero temperature and high pressure on the inactivation of Escherichia coli. It was observed that a synergistic interaction between subzero temperature and high pressure occurred in their effects on microbial inactivation.

Author: Gervais, Patrick, Moussa, Marwen, Perrier-Cornet, Jean-Marie
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Escherichia coli, Viruses, Virus inactivation, Materials at high pressures, High pressure effects

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Subjects list: Research, Physiological aspects, Observations, Cell death
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