Maximizing plasmid stability and production of released proteins in Yersinia enterocolitica

Article Abstract:

A study of the roles of temperature, calcium and pH in the expression of the 25-kDa protein YopE and other Yops produced by Yersinia enterocolitica showed that the lowest temperature at which plasma-encoded proteins are expressed in quantity is 32 degrees Celsius, while the highest calcium concentration for their full expression was 445 to 545 micromoles. pYV stability is also found to be enhanced at low pH. Results provide new approaches for enhancing enrichment, isolation and identification of pathogenic YEP strains of Y. enterocolitica from natural or clinical sources.

Research, Plasmids, Yersinia enterocolitica

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Population genetics of human, animal, and environmental Yersinia strains

Article Abstract:

The possibility of epidemiologically correlating human, animal and environmental isolates was investigated. This involved the measurement of genetic relatednessand the analysis of genetic distances among and between strains of nine Yersinia species. Bacterial isolates were subjected to phenotypic characterization, genetic analysis and statistical analysis. The different Yersinia species exhibited variable genetic distances which shows different levels of DNA homlogy among the species.

Genetic aspects, Yersinia

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A surface-focused biotinylation procedure identifies the Yersinia pestis catalase KatY as a membrane-associated but non-surface-located protein

Article Abstract:

The major surface proteins of the plaque bacterium Yersinia pestis which was identified by applying a novel surface biotinylation method followed by NeutrAvidin (NA) bead capture is presented.

Analysis, Physiological aspects, Growth, Membrane proteins, Liquid chromatography, Company growth, Structure, Yersinia pestis, Biotin

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