Nocardia sp. carboxylic acid reductase: cloning, expression, and characterization of a new aldehyde oxioreductase family

Article Abstract:

The cloning and expression of the first carboxylic acid reductase(CAR) gene, car, and the use of cloned enzyme in vitro and in vivo to reduce carboxylic acid substrates is reported. CAR from Nocardia sp. strain NRRL 5646 has an extremely wide substrate range, and it enantiospecifically reduces carboxylic acids.

Gene expression, Microbiological research, Carboxylic acids, Aldehydes

---

GTP cyclohydrolase I: purification, characterization, and effects of inhibition on nitric oxide synthase in Nocardia species

Article Abstract:

The purification and biochemical characterization of GTP cyclohydrolase I (GTPCH) from Nocardia sp. Strain NRRL 5646 is described. Results suggest that in Nocardia cells, NOS synthesis of nitric oxide is indirectly decreased by reducing the biosynthesis of an essential reducing cofactor, BH4.

Biosynthesis, Purification

---

Thiols in nitric oxide synthase-containing Nocardia sp. strain NRRL 5646

Article Abstract:

Thiol formation and detection of mycothiol (MSH)-dependent formaldehyde dehydrogenase activity in cell extracts which are relevant to the possible modulation of nitric oxide toxicity generated by Nocardia sp.strain NRRL 5646 is presented.

All Other Basic Organic Chemical Manufacturing, Industrial Organic Chemicals, Mercaptans, Environmental aspects, Thiols

Similar abstracts:

• Abstracts: Purification and characterization of a novel erythrose reductase from Candida magnoliae
• Abstracts: Isolation and biochemical characterization of two novel metagenome-derived esterases. Prospecting for novel biocatalysts in a soil metagenome
• Abstracts: Purification and characterization of an extracellular poly(L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K104-1
• Abstracts: Salinity decreases nitrite reductase gene diversity in denitrifying bacteria of wastewater treatment systems. Nitrogen control of atrazine utilization in Pseudomonas sp. strain ADP
• Abstracts: Identification and characterization of a spinal muscular atrophy-determining gene. Molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.