Nutrient utilization and transport in the thermoacidophilic archaeon Sulfolobus shibatae

Article Abstract:

Growth on a range of different carbon and nitrogen substrates was measured and the transport of nutrients into the cell was investigated to examine the growth physiology of Sulfolobus shibatae. Maltose in particular and dissacharides in general demonstrated better growth than monosaccharides. Maltose utilization was not inhibited in the presence of glucose, indicating that glucose is not the preferred substrate of S. shibatae. Two maltose transport systems were identified: a high-affinity, possible membrane-binding system and a low-affinity, proton-dependent system. Both demonstrated differential responses to treatment with 2,4-dinitrophenol and arsenate. Unlike other maltose transport systems, they were constitutive under all conditions tested and were not repressed by glucose.

Physiological aspects, Biological transport, Archaeabacteria, Archaea

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The phage-like element PBSX and part of the skin element, which are resident at different locations on the Bacillus subtilis chromosome, are highly homologous

Article Abstract:

Two unusual genetic elements reside at different locations on the Bacillus subtilis chromosome, the phage-like PBSX and skin. PBSX is found at approximately 100 degrees on the chromosome, while the skin element is found at approximately 230 degrees. Phage-like operons which are part of the skin element are highly homologous to the control and late operon regions of PBSX. Similarities between these operons in gene organization, percentage identity of the products of homologous ORFs and positioning and strengths of ribosome-binding sites for each ORF were observed.

Genetic aspects, Bacillus subtilis, Operons

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Adaptive response of the archaeon Sulfolobus acidocaldarius BC65 to phosphate starvation

Article Abstract:

The global gene expression of Sulfolobus acidocaldarius BC65 shows an adaptive response during phosphate starvation. Southern blot analysis indicates the presence of genes, homologous to the Escherichia coli genes, that participate in the phosphate starvation response system. Synthesis of some proteins increases while the production of several proteins decreases. Two-dimensional nonequilibrium pH gradient electrophoresis of cells suggests a change in the degree of phosphorylation of many proteins.

Prokaryotes, Proteins, Protein synthesis, Phosphorylation

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