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Peptide ligands that bind selectively to spores of Bacillus subtilis and closely related species

Article Abstract:

A model detection system involving a peptide ligands that bind tightly to spores of Bacillus subtilis and a standard small-molecule ligand capable of binding this spore is constructed. The results suggested that short peptides could be used as species-specific ligands and suggest that other short peptides can be isolated as specific ligands for different spores species and perhaps for any cell type.

Author: Benedek, Orsolya, Knurr, Jordan, Heslop, Jennofer, Vinson, Robert B., Botdston, Jeremy A., McAndrew, Joanne, Kearney, John F., Turnbough, Charles L., Jr
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Environmental aspects, Bacillus subtilis, Ligand binding (Biochemistry)

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Thermal inactivation of Bacillus anthracis spores in cow's milk

Article Abstract:

Decimal reduction time (time to inactivate 90% of the population) (D) values of Bacillus anthracis spores in milk ranged from 3.4 to 16.7 h at 72 degree Celsius and from 1.6 to 3.3 s at 112 degree Celsius. The calculated increase of temperature needed to reduce the D value by 90% varied from 8.7 to 11.0 degree Celsius, and the Arrhenius activation energies ranged from 227.4 to 291.3 kJ/mol.

Author: Diez-Gonzalez, Francisco, Sa Xu, Labuza, Theodore P.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Methods, Milk, Milk pasteurization

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Species-specific peptide ligands for the detection of Bacillus anthracis spores

Article Abstract:

Research describes the synthesis of peptide ligands specific to Bacillus anthracis spores and to spores from a phylogeneticlly similar variant of B. anthacis. The petide sequences are ATYPLPIR abd SLLPGLP, respectively. Data indicate that the peptides are highly specific without any false-positive binding.

Author: Williams, David D., Benedek, Orsolya, Turnbough, Charles L. Jr.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
United States, Evaluation, Design and construction, Binding proteins

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Subjects list: Research, Genetic aspects, Peptides, Spores (Bacteria), Bacterial spores, Bacillus anthracis
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