Protein kinase M, the cytosolic counterpart of protein kinase C, remodels the internal cytoskeleton of the mammalian egg during activation

Article Abstract:

A study of the different mechanisms by which intracellular signals rearrange the spatial distribution of cytoskeleton during zygote formation from the mammalian egg using pharmacological, biochemical and cytological techniques reveals that protein kinase C (PKC) and its cytosolic active equivalent PKM act in tandem during egg activation. Kinase distribution undergoes a temporal shift. PKC is present in ionophore-activated eggs in the detergent-soluble fraction while PKM is present in the sheet enriched fraction. Internal cytoskeleton of the egg is not varied by an increase in intracellular Ca2+ levels.

Physiological aspects, Protein kinases, Cytoskeleton

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Biochemical evidence for cell fusion in placentas of mouse aggregation chimeras

Article Abstract:

Analysis of the presence of glucose phosphate isomerase (GPI-1) heteropolymer in mouse placenta through a study of several mouse chimeras differing at the Gpi-1s locus reveals that fusion of two monomers GPI-1A and GPI-1B result in the heteropolymer, which is present in 37% of the chimeras studied. In the placenta, the zygotic cells fuse with each other and not with maternal cells. The constituents of the fetus and the placenta are similar and the placental constituents are distributed bimodally.

Placenta

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Regulation of dictyostelium adenylylcyclases by morphogen-induced modulation of cytosolic pH or Ca2+ levels

Article Abstract:

Experimental studies on the influence of weak acids and bases on the activities of ACA and ACG, two adenylylcyclases and cAMP secretion reveal that the pH dependence of ACA and ACG regulate the weak acid influences on their activity. Weak bases such as ammonia do not regulate cAMP concentrations, while they inhibit ACG and ACA activities.

Measurement, Adenylate cyclase, Cyclic adenylic acid, Cyclic adenosine monophosphate, Hydrogen-ion concentration, pH measurement

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