Purification of Synechocystis sp. strain PCC6308 cyanophycin synthetase and its characterization with respect to substrate and primer specificity

Article Abstract:

The properties of the enzyme cyanophycin synthetase from Synechocystis sp. strain PCC6308 are described. Cyanophycin is a multi-L-arginyl-poly [L-aspartic acid] cell inclusion in cyanobacteria that accumulates nitrogen during the exponential to stationary growth phase and disappears during balanced growth.

Microbial enzymes

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Physiological conditions conductive to high cyanophycin content in biomass of Acinetobacter calcoaceticus strain ADP1

Article Abstract:

The effects of the inorganic medium components, the initial pH, the incubation temperature, the oxygen supply, the carbon-to-nitrogen ratio, and chloramphenicol on the synthesis of cyanophycin by Acinetobacter calcoaceticus strain ADPI were studied in a mineral salts medium containing sodium glutamate and ammonium sulfate as carbon and nitrogen sources. It was found that A. calcoaticus has a high capacity for cyanaphycin synthesis and accumulation.

Genetic aspects, Biomass

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Protamylasse, a residual compound of industrial starch production, provides a suitable medium for large-scale cyanophycin production

Article Abstract:

The media containing protamylasse as the sole compound diluted only in water is investigated for their suitability in large-scale cyanophycin (CGO) production. The use of cheap residual protamylasse contributes in establishing an economically and also ecologically feasible process for the biotechnological production of CGP.

Wet corn milling, Potatoes, Potato Farming, Starch and Vegetable Fats and Oils Manufacturing, Irish potatoes, Starch & Natural Chemicals, Starch ex Corn Starch, Methods, Starch, Biosynthesis, Chemical properties, Potato

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