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Quantification of fungal hyphae in leaves of deciduous trees by automated image analysis

Article Abstract:

An optical measuring technique for fungal biomass of deciduous trees' decomposing leaves now exists. The method, in combination with the automated image analysis, is efficient for measuring the fungal biomass in heterogeneous environments. The image analysis program allows for the identification of fungal hyphae, removal of stomata from the images, and measurement of the fungal hyphae. The fungal biomass in leaves of Fagus silvatica and Quercus petraea, which is 0.2 to 0.4% of the leaf weight in the early stages, reaches a maximum in 2 to 4 weeks and then decreases.

Author: Daniel, O., Schonholzer, F., Zeyer, J.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
Methods, Microscope and microscopy, Microscopes, Microscopy, Foliar diagnosis

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Fatty acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae

Article Abstract:

Analysis of fatty acid methyl ester (FAME) profiles helps evaluate the diversity and quantity of fatty acids in 53 isolates of 24 species of glomalean fungi. FAME profiles of the citrus rootstock Carrizo citrange are compared with those of spores and endomycorrhizal roots of sudan grass. Estimation of total incidence and vesicle intensity, and clearing and staining methods reveal the correlation between mycorrhizal colonization and production of fatty acid. FAME analysis helps determine the allocation of carbon to intraradical fungal growth and lipid storage.

Author: Graham, James H., Hodge, Nancy C., Morton, Joseph B.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
Mycorrhizas, Mycorrhizae, Sorghum

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The composition and attributes of Colletotrichum truncatum spores are altered by nutritional requirements

Article Abstract:

The influence of the nutritional condition on conidiation and conidial attributes of the fungus Colletotrichum truncatum NRRL 13737, causing disease in the weed Sesbania exaltata, was reported. The carbon-to-nitrogen (CN) ratio of the medium influenced the substrate utilization, culture growth, spore yield, spore composition, biomass accumulation and cellular composition. Optimization experiments suggested that media with a CN ratio of 15:1 to 20:1 might be optimal for spore production.

Author: Jackson, Mark A., Schisler, David A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992

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Subjects list: Research, Growth, Fungi
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