Regulation of RNA polymerase through the secondary channel

Article Abstract:

A study is conducted to illustrate how regulatory factors and small molecules can exploit the secondary channel to gain access to the active site and modify the transcription properties of RNA polymerase. High-resolution crystal structure have highlighted functionally important regions in multisubunit RNA polymerases, including the secondary channel or pore, which is postulated to allow the diffusion of small molecules both into and out of the active center of the enzyme.

Enzymes, Enzyme regulation

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Crystal structure of a sigma70 subunit fragment from E. coli RNA polymerase

Article Abstract:

The domain organization of a sigma70 subunit fragment from the RNA polymerase of Escherichia coli is determined using limited proteolysis, N-terminal sequencing and mass spectrometry. The fragment's crystal structure reveals an entirely helical, V-shaped protein. The structure indicates that DNA interactions may be inhibited in the absence of RNA polymerase. Moreover, it can be used to interpret a large number of genetic and biochemical analyses.

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Structural basis for bacterial transcription-coupled DNA repair

Article Abstract:

The 3.2 A-resolution X-ray crystal structure of Escherichia coli transcription-repair coupling factor (TRCF) is discussed. A compact arrangement of eight domains, a translocation module and a region of structure similarity to UvrB is observed in the structure.

New York, Analysis, Physiological aspects, DNA repair, Genetic transcription, Transcription (Genetics), Crystals, Crystal structure

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