Screening actinomycetes for extracellular peroxidase activity

Article Abstract:

The horseradish peroxidase-based enzyme-linked immunosorbent assay based on chemiluminescence analysis with an Amerlite analyzer effectively detects the production of extracellular peroxidase in actinomycetes. An analysis of 39 strains by this method identified five strains producing a large amount of peroxidase. Spectrophotometric assay of Streptomyces strain EC 22 and S. thermoviolaceus shows that detection of 2,4-dichlorophenol oxidation is the most sensitive and reproducible method for determining peroxidase activity.

Usage, Observations, Streptomyces, Enzyme-linked immunosorbent assay, Spectrophotometry

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Characterization of laccases and peroxidases from wood-rotting fungi (Family Coprinaceae)

Article Abstract:

The basidiomycetes Panaeolus sphinctrinus, P. Papilionaceus and Coprinus friesii elaborate laccases and peroxidases. These enzymes are purified and analyzed by substrate specificity, enzymatic activity at different pH values and temperature sensitivity and specific inhibitors that alter their kinetics are identified. Polyacrylamide gel electrophoresis of native enzymes as well as isoelectric focusing revealed that these basidiomycetes produce isoenzymes.

Physiological aspects, Microbial enzymes, Wood-decaying fungi

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Extracellular heme peroxidases in actinomycetes: a case of mistaken identity

Article Abstract:

Researchers report that the heme peroxidases allegedly secreted by actinomycetes are actually zinc coproporphyrin. The proteins have a heme-like optical spectrum that can be incorrectly assigned to an iron-containing porphyrin.

Metalloproteins, Actinomycetales

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