Self-transmissible mercury resistance plasmids with gene-mobilizing capacity in soil bacterial populations: influence of wheat roots and mercury addition

Article Abstract:

Research was conducted to examine self-transmissible mercury resistance plasmids with gene-mobilizing capacity in soil bacterial populations. Exogenous plasmid isolation was utilized to derive transferable plasmids from soil bacteria through the use of mercury resistance as the selectable marker. Wheat roots and mercury were studied as experimental variables since the incidence of plasmids in soil bacteria may be influenced by soil ecological factors and selection pressure. Results suggest that the addition of various concentrations of HgCl2 affected total bacterial counts.

Genetic aspects, Soil microbiology, Population genetics, Mercury compounds, Wheatgrasses, Wheatgrass

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Mobilization of a recombinant IncQ plasmid between bacteria on agar and in soil via cotransfer or retrotransfer

Article Abstract:

The IncQ plasmid is a non-self-transmissible plasmid which is widely used as a vector for genetic engineering purposes and can be mobilized by plasmids of different incompatibility groups. An investigation was conducted to determine if a genetically engineered IncQ plasmid, pSKTG, can be mobilized in vitro in sterile and nonsterile soils. The results showed that pSKTG could be mobilized only in the presence of a self-transmissible plasmid. However, pSKTG could be transferred in the natural soil environment, probably through the activity of natural elements.

Environmental aspects, Microbial genetic engineering, Recombinant molecules

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Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate

Article Abstract:

Researchers have discovered a bacterium from costal marine sediment that contains a 101-kb plasmid encoding five different agarases. Microscilla sp. strain PRE1 that lost the plasmid was unable to degrade agar.

Biodegradation, Marine bacteria

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