Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots

Article Abstract:

A polymerase chain reaction primer was designed to amplify small subunit rRNA the arbuscular endomycorrhizal fungus directly from fungal and plant tissues. This was done by amplification and direct sequencing of the SSU gene from Glomus intraradices, Gigaspora margarita and E. pisiformis. The primer was found to be specific for arbuscular endomycorrhizal fungi and allowed the analysis of a specific gene or gene fragment from a minute amount of starting materials.

Fungi, Identification and classification

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Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein

Article Abstract:

A study was conducted to determine the effect of adding bovine serum albumin (BSA) or T4 gene protein (gp32) to polymerase chain reaction. Neither proteins were able to significantly relieve interference in the presence of minimum inhibitory levels of bile salts, bilirubin, EDTA, NaCl, sodium dodecly sulfate or Triton X-100. Neither protein significantly affected amplification in the absence of inhibitors.

Polymerase chain reaction

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Analysis of the endophytic actinobacterial population in the roots of wheat (Triticum aestivum L.) by terminal restriction fragment length polymorphism and sequencing of 16S rRNA clones

Article Abstract:

Endophytic populations have been isolated and characterized primarily by cultivation-based methods. Due to limitations of culture-based methods, molecular methods of community analysis need to be employed.

Bacteria, Microbiological research, Endophytes

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