Strategies for achieving high-level expression of genes in Escherichia coli

Article Abstract:

Efficient gene expression in Escherichia coli needs a strong and precisely regulated promoter at the 5' region of the gene and an efficient transcription terminator positioned at the 3' end. A wide choice of these elements is available to suit the individual need for the expression of different genes. Host strains with low proteolytic activity are also available. Better knowledge of protein secretion by the bacteria can help in secretion of the expressed protein into the medium. Varying the codon usage and use of chaperones can improve gene expression.

Research, Usage, Gene expression, Promoters (Genetics), Codon, Codons

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Central roles of small GTPases in the development of cell polarity in yeast and beyond

Article Abstract:

The molecular mechanisms underlying the development of cell polarity in budding yeast is reviewed with particular focus on the roles of small GTPases. The key signaling pathways that regulate cell polarization during the mitotic cell cycle and during mating is discussed.

Cell cycle, Yeast fungi, Yeasts (Fungi), Guanosine triphosphatase, GTPases

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Allosteric regulation of catalytic activity: Escherichia coli aspartate transcarbamoylase versus yeast chorismate mutase

Article Abstract:

This review focuses on the major investigations into the baker's yeast made during the last decade. The comparison of the established aspartate transcarbamoylase model system with the catalytic and regulatory features of E. coli has been performed and analyzed.

Germany, Statistical Data Included, Yeast, Yeast (Food product), Microbiological research, Aspartate

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