The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos

Article Abstract:

A study shows that green fluorescent protein (GFP) gene product is easily visible in zebrafish when propelled by a strong enhancer-promoter injected with plasmid DNA and in stable transgenic lines into embryos. A single copy of the transgene is adequate to confer detectable fluorescence in hemizygous embryos F1 and F2. The study used three different expression constructs with each employing a changed ef1 alpha enhancer-promoter to generate 12 transgenic lines.

Fishes, Embryology, Animal embryology

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Extracellular matrix protein fibulin-2 is expressed in the embryonic endocardial cushion tissue and is a prominent component of valves in adult heart

Article Abstract:

Immunofluoresence studies reveal that fibulin-1 and -2 are significantly codistributed throughout the development of the endocardial cushion tissue (ECT) and are expressed in the heart valve in the adult stage. A comparative study suggests that fibulin-2 is a better marker than fibulin-1 for the ECT. ECT development and the tensile strength of the cardiac values are influenced by these two fibulins.

Heart valves, Endocardial cushion defects

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CNBP regulates forebrain formation at organogenesis stage in chick embryos

Article Abstract:

A study examines the role of cellular nucleic acid binding protein in the formation of forebrain in the embryos of chick.

United States, Usage, Growth, Company growth, Chick embryo, Binding proteins, Organogenesis

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