The Caenorhabditis elegans spe-6 gene is required for major sperm protein assembly and shows second site non-complementation with an unlinked deficiency

Article Abstract:

The crawling motility of Caenorrhabditis elegans spermatozoa is dependent on its thin cytoskeletal filaments made from a cytoskeletal protein, the major sperm protein (MSP). Studies on the effects on the viability and failure of filament assembly of the spe-6 gene led to the conclusion that the gene product is required for the normal sperm development and MSP localization. A concentration-dependent interaction between spe-6 gene and the sperm-specific gene products, eDf18 and eDf19 was found necessary for MSP assembly.

Cytoskeletal proteins, Spermatozoa, Sperm motility

---

Isogenic strain construction and gene mapping in Candida albicans

Article Abstract:

Researchers designed a genotypic screen that allowed determination of a heterozygous recessive mutation at the URA 3 locus to make up for the limitations in the genetic manipulation of candida albicans that are the result of its diploid genome and a sexual life cycle. The 18 bp recognition sequence of the endonuclease I-Sce I was integrated into the hisG repeats to allow the physical mapping of disrupted genes. Site-specific cleavage of the chromosome with I-SceI indicated the placement of the combined sequences.

Candida albicans

---

Similar abstracts:

• Abstracts: Overexpression of Candida albicans secretory aspartyl proteinase 2 and its expression in Saccharomyces cerevisiae do not augment virulence in mice
• Abstracts: Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis. Ironing out the kinks: splicing and translation in bacteria
• Abstracts: A model for antagonistic pleiotropic gene action for mortality and advanced age. Linkage analysis of X-linked cone-rod dystrophy: localization to Xp11.4 and definition of a locus distinct from RP2 and RP3
• Abstracts: The dilemma of plants: to grow or defend. Community heterogeneity and the evolution of interactions between plants and insect herbivores
• Abstracts: Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in the yeast

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.