The TATA-box binding protein of Entamoeba histolytica: cloning of the gene and location of the protein by immunofluorescence and confocal microscopy

Article Abstract:

A study was conducted to analyze the TATA-box binding protein of Entamoeba histolytica. A DNA fragment from E. histolytica was amplified by polymerase chain reaction (PCR) using primers determined from the Acanthamoeba castellanii consensus TATA-box binding protein amino acid sequence. The copy number of the Ehtbp gene was then obtained by Southern blot experiments. In addition, the cellular location of the gene was analyzed by in situ PCR experiments.

Usage, Polymerase chain reaction, Entamoeba

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Fructose and mannose metabolism in Aeromonas hydrophila: identification of transport systems and catabolic pathways

Article Abstract:

The uptake of fructose and mannose in the opportunistic pathogen Aeromonas hydrophila is investigated. A. hydrophila has a fructose-specific and a mannose-specific transport system via separate phosphotransferase systems (PTS). Both fructose PTS and mannose PTS in A. hydrophila exhibit cross reactivities with other PTS from various organisms. The metabolic pathway undergone by these substrates upon entering the cell are discussed.

Physiological aspects, Gram-negative bacteria, Microbial metabolism

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