TnpA trans-activates methylated maize suppressor-mutator transposable elements in transgenic tobacco

Article Abstract:

The influence of individual CaMV 35S promoter-driven cDNAs for tnpA, tnpB, tnpC and tnpD was analyzed to determine if element-encoded gene products can reactivate an inactive element in transgenic tobacco. Results showed that introduction of tnpA cDNA reactivated the inactive resident Suppressor- mutator (Spm) element, while tnpB, tnpC and tnpD cDNAs did not. In addition, decreased methylation of methylation-sensitive restriction sites near the transcription sites resulted with the reactivation of the inactive Spm element. The continued presence of tnpA cDNA also needed for the maintenance of the reactivated Spm.

Genetic aspects, Tobacco

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Early elements in gastropod neurogenesis

Article Abstract:

New findings about the processes underlying gastropod neurogenesis are presented. Immunocytochemical studies suggest that the first elements of the gastropod nervous system develop earlier than previously reported. The somata are location posteriorly within the embryo and outside the future central nervous system. Anteriorly directed fibers from these cells form a scaffold which later gives rise to the central ganglia and interconnecting pathways.

Physiological aspects, Nervous system, Gastropoda, Gastropods

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PRECISE: Software for Prediction of cis-Acting Regulatory Elements

Article Abstract:

Benefits to using PRECISE include a user-friendly interface and the availability of empirical motif frequency tables for filtering discovered motifs. The software is applied to two case studies.

Facilities & equipment, Evaluation, Product/Service Evaluation, Equipment and supplies, Science, Genetic research, Scientific software, PRECISE (Scientific software)

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