Transcription activation at class II CAP-dependent promoters: two interactions between CAP and RNA polymerase

Article Abstract:

Transcription activation by Escherichia coli Class II catabolite activator protein (CAP) involves a second activating region for Class II CAP-dependent promoters. Random mutagenesis of the gene encoding CAP did not alter the transcription activation of Class I CAP-dependent promoters and deoxyribonucleic acid binding. The activation of Class II-dependent CAP promoters is also characterized by the interaction of the ribonucleic acid polymerase alpha subunit N-terminal domain and specific amino acids in the Class II-specific activating region of CAP.

Genetic aspects, RNA polymerases

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Chromatin, TAFs, and a novel multiprotein coactivator are required for synergistic activation by Sp1 and SREBP-1a in vitro

Article Abstract:

Sp1, a promoter selectivity factor, often works with other enhancer-binding proteins to bring on transcription. Synergy seen in vivo between the sterol-regulated factor SREBP-1a and Sp1 at the low-density lipoprotein receptor (LDLR) promoter has been achieved in vitro. A novel multiprotein coactivator, TBP-associated factors (TAFs), and chromatin are necessary in vitro for synergistic activation by SREBP-1a and Sp1. A human cellular fraction with the coactivator CBP in it is able to support high levels of activation that is chromatin dependent.

Observations, Chromatin, Proteins, Cellular signal transduction, Genetic regulation, Cell receptors

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TAFs and TFIIA mediate differential utilization of the tandem Adh promoters

Article Abstract:

A study analyzing the activity of the D. melanogaster alcohol dehydrogenase (Adh) in order to determine the mechanism of promoter selectivity finds that the selective promoter utilization is controlled by distinct initiator elements, TAFs and TFIIA. Reconstitution of Adh transcription with purified component needs a specific TBP-TAF complex which in concert with TFIIA, catalyzes differential Adh promoter transcription. Further analysis reveals that TAFII150 is required for judgment between distal and proximal promoters.

Research, Drosophila, Alcohol dehydrogenase, Alcohol dehydrogenases

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