UniFrac: A new phylogenetic method for comparing microbial communities

Article Abstract:

A new phylogenetic method, called UniFrac that measures the distance between microbial communities based on phylogenetic information is described. The results have shown that UniFrac provides a new way of characterizing microbial communities, using the environmental rRNA sequences, and allows quantitative insight into the factors that underlie the distribution of lineages among environments.

Methods, Phylogeny

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Construction of in-frame aroA deletion mutants of Mannheimi haemolytica, Pasteurella multocida, and Haemophilus somnus by using a new temperature-sensitive plasmid

Article Abstract:

A temperature-sensitive (TS) plasmid is generated from the endogenous streptomycin resistance plasmid of Mannheimia hemolytica and used to engineer in-frame aroA deletion mutants of Mannheimi haemolytica, Pasteurella multocida, and Haemophilus somnus. The system used represents a broadly applicable means for generating unmarked mutants of Pasteurellaceae species.

Genetic aspects, Plasmids, Hemophilus infections

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Chlorophenol production by anaerobic microorganisms: transformation of a biogenic chlorinated hydroquinone metabolite

Article Abstract:

Mixed microbial communities from estuarine sediment and a pure culture of Desulfitobacterium sp. strain PCE1 were studied for determining a new route of biodegradation, which ended with the increase in the amount of chlorophenols. Results suggest that the chlorophenols could be formed naturally by the anaerobic degradation of the chlorinated fungal metabolite.

Manufacturing processes, Causes of, Production processes, Chlorophenols, Fungal antigens

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